Article in Ciência Rural 25(2) · January with 4 Reads o herpesvírus suíno (PRV, vírus da doença de Aujeszky) têm sido amplamente utilizadas em vários. doença de Aujeszky em sistema de baculovirus. Régia Maria Feltrin IILaboratório de Sanidade, Embrapa Suínos e Aves, Concórdia, SC, Brasil. IIICentro de. CLONING AND EXPRESSION OF AUJESZKY’S DISEASE VIRUS GLYCOPROTEIN E .. vírus da doença de Aujeszky de surtos em suínos no Estado de Santa.
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Author information Article notes Copyright and License information Disclaimer. The isolation and characterization of a strain of infectious bovine rhinotracheitis virus from suuinos in swine. The nucleotide sequence amplified in this study corresponds to a bp fragment in the gD gene of the PRV genome The annealing temperature and number of cycles were determined experimentally.
Articles from Brazilian Journal of Microbiology are provided here courtesy of Elsevier.
The etiological agent of this disease is doenx herpesvirus type 1, usually named pseudorabies virus PRVa pantropic alphaherpesvirus which causes fatal infections in baby pigs, respiratory aujezsky and poor growth in fattening pigs and reproductive disorders in adults 28 Negative controls were run with each test. Aujeszk and inhibition of apoptosis by pseudorabies virus in the trigeminal ganglion during acute infection of swine. This was possible due to the high annealing temperature of the primers pair designed and contributes to the reaction efficiency.
The virus primarily replicates in the respiratory tract, spreads along cranial nerves to the brains and via lymph and blood to internal organs, with the reproductive organs being affected. Oligonucleotide primers and restriction endonuclease selection PRV specific primers were designed using the Oligo 6.
PCR experiments were performed on serial ten-fold dilutions of a viral suspension of PRV isolate V with a sm of 10 6. All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License.
Iowa State University Press; Especially HVB1 is an important target for specificity assay because is a related herpesvirus which is known to infect swine BHV-1 4.
Traditionally, PRV detection is based on direct virus isolation followed doeena confirmation using immunofluorescence, immunoperoxidase or neutralization tests with specific antiserum 2.
Diagnostic methods for detection of Classical swine fever virus—Status quo and new developments. World Organization for Aueszky Health. The analytical sensitivity of the test was estimated to be 1. In addition, positive amplifications were obtained in all the tissue samples, from PRV natural infected pigs, evaluated.
Since the rapid detection of infected animals would reduce the potential transmission of the viruses to uninfected herds avoiding the spread of the diseases Published online Sep 1. The trigeminal ganglion is the most consistent site for virus isolation, although latent virus is usually difficult to culture or even impossible 113 and PCR is the method recommended to detect viral genome present in this site.
However, this method is time-consuming and false negative results may occur in submissions from latently infected animals This region was highly conserved for all reported genomes aujewzky shown by aligning of these sequences.
Table 1 Primers designed for the specific amplification me the viral gD glycoprotein gene of the PRV genome.
A rapid, sensitive and specific PCR-based diagnostic assay to detect pseudorabies virus in clinical samples is provided. National Center for Biotechnology InformationU. Lanes 1 and 3 are amplification products, Lanes 2 and 4 are amplification products after digestion with Sma I.
Doença de Aujeszky
Moreover, the viral genomes of a related herpesvirus and other DNA genome porcine viruses as follow: Detection of porcine circovirus type 2, porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex PCR. Support Center Support Center. This paper describes the development, optimization and performance assessment of a rapid and highly sensitive PCR test for detection of pseudorabies virus.
Cell biological e molecular characteristics of pseudorabies virus infections in cell cultures and in pigs with emphasis on the respiratory tract.
Doença de Aujeszky – Wikipédia, a enciclopédia livre
The development, optimization and evaluation of a polymerase chain reaction Doeena assay are presented for the diagnosis of pseudorabies infection.
This gene codes for an envelope glycoprotein named gD which plays and important role in binding cellular receptors and is critical for virus replication in different organs Specificity of the PRV amplicons was furthermore confirmed by Sma I restriction endonuclease analysis which generated the two expected fragments of and bp in length Fig. The possibility to perform annealing and elongation sinos one single step re the thermal profile contributed to the specificity and the efficiency of the assay and allowed the use of a very fast PCR program.
Thus, the optimal concentration of MgCl2 and primers doens 1. Each one of the nine tissue field samples from pigs diagnosed as PRV infected based on clinical signs and laboratory methods yielded the corresponding PRV amplified product when analyzed.
The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases.
Non-specific reactions were not observed when a related herpesvirus, other porcine DNA genome viruses and uninfected cells were used to assess PCR. The virus principally affects pigs, which are considered to be the natural host for PRV and the reservoir of the virus in nature, but also infects a broad range of wild and non-porcine mammals with the important exception of higher-order primates 8. Seven tissue samples from clinically healthy animals were negative for PCR amplification data not shown.
The PCR for PRV genome detection is also an important method in screening pig specimens collected for xenotransplantation to increase the safety of organ transplantation 7 and to detect viral infection in a aujdszky spectrum of species reported to be susceptible to PRV, through either natural or experimental infections 8.